Archives of Public Health

Vol. 12 No. 1 (2020): Archives of Public Health
Clinical Science

Increased level of IL-8 in amniotic fluid in early second trimester linked with preterm pregnancies

pdf (македонски јазик)
  • Katerina Nikoloska,
  • Iva Malahova- Gjoreska,
  • Ivo Kaev,
  • Aleksandar Petlickovski
Katerina Nikoloska
University clinic of gynecology and obstetrics, Skopje, Republic of North Macedonia
Iva Malahova- Gjoreska
University clinic of gynecology and obstetrics, Skopje, Republic of North Macedonia
Ivo Kaev
University clinic of gynecology and obstetrics, Skopje, Republic of North Macedonia
Aleksandar Petlickovski
Institute for immunology and human genetics; Medical Faculty, Ss Ciril and Methodius, Skopje, Republic of North Macedonia
DOI: https://doi.org/10.3889/aph.2020.4472

Published 2020-02-20

Keywords

  • preterm birth,
  • amniotic fluid,
  • IL-8,
  • amniocentesis,
  • infection

How to Cite

1.
Nikoloska K, Malahova- Gjoreska I, Kaev I, Petlickovski A. Increased level of IL-8 in amniotic fluid in early second trimester linked with preterm pregnancies. Arch Pub Health [Internet]. 2020 Feb. 20 [cited 2024 Apr. 18];12(1):5-13. Available from: https://id-press.eu/aph/article/view/4472
Copyright Notice

Abstract

Cytokines (IL-1, IL-6, IL-8, TNF- alfa) are of crucial importance during pregnancy; they are produced by the placenta in the amniotic fluid and they are elevated in case of intrauterine inflammation. The аim of the study was to prove the ratio between the increased IL-8 in the amniotic fluid in the beginning of the second trimester (16-22 g.w.) and premature birth (< 36.6 g.w.). Material and methods: This was a prospective study that included 150 pregnant patients that had clinical indication for amniocentesis (advanced mother’s age, abnormal test of PRISCA I, suspicious anomalies of the fetus, virus infection or mother’s wish). They all gave a signed consent on being informed about the aims of the study, and following the protocol, they were analyzed and examined i.e. all patients underwent ultrasound examination, vaginal cervicometry. Five ml. of amniotic fluid during the process of amniocentesis was taken for the purpose of the study. All patients were followed until they gave birth, and the exact week of gestation was noted and compered with the IL-8 level. Results: All 150 patients were in the period of 16th-22nd gestational weeks. Twenty of the total of 150 patients had preterm delivery. A total of 139 patients conceived naturally and 9 patients underwent in vitro fertilisation (IVF) and embryo transfer (ET). In those with IVF and ET, 3 had preterm birth. 80% of patients that had preterm birth had increased IL- 8 levels. Median cervical length in those who gave birth at term was 32.1 mm and in those who gave preterm birth was 30.7mm. Conclusion: The study has confirmed the reason for examining cytokines as a method of discovering asymptomatic changes in patients who would give a premature birth.

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References

  1. Rehbinder EM, Lí¸drup Carlsen KC, Staff AC, et al. Is amniotic fluid of women with uncomplicated pregnancies free of bacteria? Am J Obstet Gynecol 2018;169:805–16.
  2. Baud O, Emilie D, Pelletier E, Lacaze-Masmonteil T, Zupan V, Fernandez H, Dehan M, Frydman R, Ville Y. Amniotic fluid concentrations of interleukin-1β, interleukin-6 and TNF-α in chorioamnionitis before 32 weeks of gestation: histological associations and neonatal outcome. Brit J Obstet Gynaecol 1999; 106: 72–77.
  3. Bulletins-Obstetrics ACoP. ACOG practice bulletin no. 80: premature rupture of membranes. Clinical management guidelines for obstetriciangynecologists. Obstet Gynecol 2007;109:1007–19.
  4. Biggio JR, Ramsey PS, Cliver SP, Lyon MD, Goldenberg RL, Wenstrom KD. Midtrimester amniotic fluid matrix metalloproteinase-8 (MMP-8) levels above the 90th percentile are a marker for subsequent preterm premature rupture of membranes. Am J Obstet Gynecol 2005;192:109–13.
  5. Apuzzio J, Chan Y, Al-Khan A, Illsley N, Kim PL, Vonhaggen S. Second-trimester amniotic fluid interleukin-10 concentration predicts preterm delivery. J Matern Fetal Neonatal Med 2004;15:313–17.
  6. Yoon B, S Oh, R Romero, S Shim, S Han, J Park, J Jun. An elevated amniotic fluid matrix metalloproteinase-8 level at the time of genetic amniocentesis is a risk factor for preterm delivery. Am J Obstet Gynecol 2001; 185: 1162.
  7. Garite TJ, Freeman RK. Chorioamnionitis in the preterm gestation. Obstet Gynecol 1982; 59: 539.
  8. Medda E, Donati S, Spinelli A, Di Renzo GC, EUROPOP group: Genetic amniocentesis: a risk factor for pretermdelivery? Eur J Obstet Gynecol 2003; 110:153-159.
  9. Suzuki Y, Yamamoto T, Kojima K, Tanemura M, Tateyama H, Suzumori K. Evaluation levels of cytokines in amniotic fluid of women with intrauterine infection in the early second trimester. Fetal Diagn Ther 2006;21:45–50.
  10. Saito S, Kasahara T, Kato Y, Ishihara Y, Ichijo M. Elevation of amniotic fluid interleukin 6 (IL-6), IL-8 and granulocyte colony stimulating factor (G-CSF) in term and preterm parturition. Cytokine 1993;5:81–8.
  11. Romero R, Yoon BH, Mazor M, Gomez R, Gonzalez R, Diamond MP, et al. A comparative study of the diagnostic performance of amniotic fluid glucose, white blood cell count, interleukin-6, and gram stain in the detection of microbial invasion in patients with preterm premature rupture of membranes. Am J Obstet Gynecol 1993;169:839–51.
  12. Romero R, Yoon BH, Mazor M, Gomez R, Diamond MP, Kenney JS, et al. The diagnostic and prognostic value of amniotic fluid white blood cell count, glucose, interleukin-6, and gram stain in patients with preterm labor and intact membranes. Am J Obstet Gynecol 1993;169:805–16.
  13. Yoon BH, Romero R, Kim CJ, Jun JK, Gomez R, Choi JH, et al. Amniotic fluid interleukin-6: a sensitive test for antenatal diagnosis of acute inflammatory lesions of preterm placenta and prediction of perinatal morbidity. Am J Obstet Gynecol 1995;172:960–70.
  14. Potter NT, Kosuda L, Bigazzi PE, Fleming AD, Vintzileos AM, Homon C, Salafia CM. Relationships among cytokines (IL-1, TNF, and IL-8) and histologic markers of acute ascending intrauterine infection. J Matern-Fetal Med 1992;1:142–147.
  15. Yoon BH, Romero R, Kim CJ, Jun JK, Gomez R, Choi JH, Syn HC. Amniotic fluid interleukin-6: a sensitive test for antenatal diagnosis of acute inflammatory lesions of preterm placenta and prediction of perinatal morbidity. Am J Obstet Gynecol 1995;172:960 – 970.
  16. Hitti J, Tarczy-Hornoch P, Murphy J, Hillier SL, Aura J, Eschenbach DA. Amniotic fluid infection, cytokines, and adverse outcome among infants at 34 weeks' gestation or less. Obstet Gynecol 2001; 98:1080 – 1088.
  17. Yoon BH, Romero R, Moon JB, Shim SS, Kim M, Kim S, Jim JK. Clinical significance of intra-amniotic inflammation in patients with preterm labor and intact membranes. Am J Obstet Gynecol 2001;185:1130 – 1136.
  18. Martinez E, Figueroa R, Garry D, Visintainer P, Patel K, Verma U, Sehgal PB, Tejani N. Elevated amniotic fluidinterleukin-6 as a predictor of neonatal ceriventricular leukomalacia and intraventricular hemorrhage. J Matern Fetal Invest 1998;8:101– 107.
  19. Yoon BH, Jun JK, Romero R, Park KH, Gomez R, Choi JH, Kim IO. Amniotic fluid inflammatory cytokines (interleukin-6, interleukin-1b, and tumor necrosis factor-a), neonatal brain white matter lesions, and cerebral palsy. Am J Obstet Gynecol 1997;177:19–26.
  20. Yoon BH, Romero R, Kim CJ, Koo JN, Choe G, Syn HC. High expression of tumor necrosis factor-a andinterleukin-6 in periventricular leukomalacia. Am J Obstet Gynecol 1997;177:406 – 411.
  21. Gervasi MT, Romero R, Bracalente G, Erez O, Dong Z,Hassan SS, et al. Midtrimester amniotic fluid concentrations of interleukin-6 and interferon-gamma-inducible protein-10: evidence for heterogeneity of intra-amniotic inflammation and associations with spontaneous early (<32 weeks) and late (>32 weeks) preterm delivery. J Perinat Med 2012;40:329–43.
  22. Bamberg C, Fotopoulou C, Thiem D, Roehr CC,Dudenhausen JW, Kalache KD. Correlation of midtrimester amniotic fluid cytokine concentrations with adverse pregnancy outcome in terms of spontaneous abortion, preterm birth, and preeclampsia. J Matern Fetal Neonatal Med. 2012;25:812–17.
  23. Himaya E, Rhalmi N, Girard M, Tetu A, Desgagne J, Abdous B, et al. Midtrimester intra-amniotic sludge and the risk of spontaneous preterm birth. Am J Perinatol.2011;28:815–20.
  24. Thomakos N, Daskalakis G, Papapanagiotou A, Papantoniou N, Mesogitis S, Antsaklis A. Amniotic fluid interleukin-6 and tumor necrosis factor-alpha at mid-trimester genetic amniocentesis: relationship to intraamniotic microbial invasion and preterm delivery. Eur J Obstet Gynecol Reprod Biol 2010;148:147–51.
  25. Lee J, Romero R, Dong Z, Xu Y, Qureshi F, Jacques S, et al. Unexplained fetal death has a biological signature of maternal anti-fetal rejection: chronic chorioamnionitis and alloimmune anti-human leucocyte antigen antibodies. Histopathology 2011; 59:928– 38.
  26. Lee J, Romero R, Xu Y, Kim JS, Park JY, Kusanovic JP, et al. Maternal HLA panel-reactive antibodies in early gestation positively correlate with chronic chorioamnionitis: evidence in support of the chronic nature of maternal anti-fetal rejection. Am J Reprod Immunol 2011; 66:510–26.
  27. Lee J, Romero R, Xu Y, Kim JS, Topping V, Yoo W, et al. A signature of maternal anti-fetal rejection in spontaneous preterm birth: chronic chorioamnionitis, anti-human leukocyte antigen antibodies, and C4d. PLoS One. 2011;16: 806-36.
  28. Madan I, Romero R, Kusanovic JP, Mittal P, Chaiworapongsa T, Dong Z, et al. The frequency and clinical significance of intra-amniotic infection and/or inflammation in women with placenta previa and vaginal bleeding: an unexpected observation. J Perinat Med 2010; 38:275– 9.
  29. Malamitsi-Puchner A, Vrachnis N, Samoli E, Baka S, Hassiakos D, Creatsas G. Elevated second trimester amniotic fluid interferon gamma-inducible T-cell alpha chemoattractant concentrations as a possible predictor of preterm birth. J Soc Gynecol Invest 2006; 13:25– 9.
  30. Malamitsi-Puchner A, Vrachnis N, Samoli E, Baka S, Iliodromiti Z, Puchner KP, et al. Possible early prediction of preterm birth by determination of novel proinflammatory factors in midtrimester amniotic fluid. Ann N Y Acad Sci 2006; 1092:440– 9.
  31. Mandar R, Livukene K, Ehrenberg A, Smidt I, Raukas E, Kask V, et al. Amniotic fluid microflora in asymptomatic women at mid-gestation. Scand J Infect Dis. 2001; 33:60– 2.