Улогата на медицинската сестра во Mакедонската банка за хумана ДНК

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Elena Cvetkovska
Mirko Spiroski
http://orcid.org/0000-0001-5559-7981

Апстракт

ОСÐОВÐ: Банка за хумана ДÐК (хДÐÐМКД) претÑтавува Ñкладирање на примерок на ДÐК од поединецот, како и нејзино безбедно и долгорочно чување Ñо цел да Ñе обезбеди примерок на генетÑки материјал за медицинÑки и научни иÑтражувања. МакедонÑката банка за хумана ДÐК датира од 2000 година и во неа Ñе дефинирани неколку проектни полиња. МедицинÑката ÑеÑтра како дел од Ñтручниот тим извршува многу активноÑти Ñо кои учеÑтвува во иÑтражувачката дејноÑÑ‚ на хДÐÐМКД.

ЦЕЛ: Целта е да Ñе презентира улогата на мединцинÑката ÑеÑтра во хДÐÐМКД во Ñите Ñегменти во кои таа зема учеÑтво почнувајќи од земањето на пиÑмена ÑоглаÑноÑÑ‚, земање на примероци, ДÐК екÑтракција и Ñкладирање на иÑтата.

ÐœÐТЕРИЈÐЛ И МЕТОДИ: Примероците на ДÐК Ñе земени од доброволни дарители по претходно потпишана ÑоглаÑноÑÑ‚, доброволно и без притиÑок. Поединците Ñе информирани во Ð¾Ð´Ð½Ð¾Ñ Ð½Ð° видот на иÑтражувањата што ќе Ñе вршат, аранжманите за приÑтап и Ñподелување на Ñкладираните примероци и времетраењето на чување на примероците. ДÐК е изолирана од периферна крв Ñо фенол-хлороформ метод за екÑтракција. Квалитетот и квантитетот на изолираната геномÑка ДÐК е определен Ñо 260/280 УВ Ñпектрофотометар Ñо GENEQUANT pro RNA/DNA калкулатор. ИнтактноÑта на изолираната ДÐК е одредена Ñо електорфореза на ДÐК на агарозен гел по што иÑтата Ñе аликвотира во нормализирана концентрација и Ñе Ñкладира во замрзнувач Heraus Sepatech на -80оЦ.

РЕЗУЛТÐТИ: Во хДÐÐМКД Ñе дефинирани три проектни облаÑти: антропологија, неÑродни пациенти и Ñродни пациенти. ЛабораториÑкиот код за ДÐК примероците што Ñе чуваат во МакедонÑката банка за хумана ДÐК е MKDSPI. Вкупниот број на примероци кои Ñе Ñкладирани во МакедонÑката банка за хумана ДÐК до крајот на 2010 година е 5252 примероци – 1575 во антропологијата, 2186 неÑродни пациенти и 1491 Ñродни пациенти.

ЗÐКЛУЧОК: МедицинÑката ÑеÑтра има целокупно учеÑтво во Ñобирање на Ñите важни информации за генетÑките иÑтражувања како земање на Ñемејна иÑторија, медицинÑки информации и доброволна ÑоглаÑноÑÑ‚ од Ñтрана на дарителите. При тоа мора да ги применува етичките приципи за одржување на приватноÑта и доверливоÑта на генетÑки информации. Преку правилно земање и Ñоодветно одбележување на примероците крв таа обезбедува точноÑÑ‚ на резултатите добиени од генетÑките иÑтражувања. ПретÑтавува неопходен дел од тимот на хДÐÐМКД заради што треба да има големо иÑкуÑтво и знаење од облаÑта на генетиката и медицинÑката етика.

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Cvetkovska, E., & Spiroski, M. (2015). Улогата на медицинската сестра во Mакедонската банка за хумана ДНК. Македонско медицинско електронско списание, 2015, 1–10. https://doi.org/10.3889/mmej.2015.50011
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